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Journal
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Biotechnik
/
3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
/
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal
Biotechnik
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening
DOI:
10.3791/200259-v
•
03:44 min
•
May 19, 2023
•
Lisa A. Krattiger
,
Maria Mitsi
,
Benjamin R. Simona
,
Martin Ehrbar
1
Department of Obstetrics
,
University Hospital Zurich, University of Zurich
,
2
Ectica Technologies AG
Tags
3D Stromal-endothelial Cells
Osteogenic Differentiation
Alkaline Phosphatase Activity
HBM-MSCs
GFP-HUVECs
BCIP/NBT Substrate
Cell Lysates
ALP Activity Quantification
DNA Quantification
Mesenchymal Stem Cells
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Verwandte Videos
Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Establishing the 3D Stromal Monoculture
Establishing the 3D Stromal-Endothelial Cell Co-Culture
Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture
Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity
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