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Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks

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Isolation and Normalization of DNA Containing Crosslinked Proteins

JoVE Journal
生化学

This content is Free Access.

JoVE Journal 生化学

Isolation and Normalization of DNA Containing Crosslinked Proteins

Isolation and Normalization of DNA Containing Crosslinked Proteins

Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks

DOI:

10.3791/200209-v

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02:49 min

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April 21, 2023

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Yilun Sun

¹Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health (NIH)

タグ

DNA Crosslinked Proteins Isolation Normalization Ubiquitylation SUMOylation PBS DNAzol Ethanol Nucleic Acid Centrifugation Pellet DNA Concentration UV-Vis Spectrometer Micrococcal Nuclease Calcium Micrococcal Nuclease Reaction Buffer

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