Journal
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生物化学
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Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks
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Isolation and Normalization of DNA Containing Crosslinked Proteins
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Isolation and Normalization of DNA Containing Crosslinked Proteins

Isolation and Normalization of DNA Containing Crosslinked Proteins

Measuring Ubiquitylated, SUMOylated, and ADP-Ribosylated DNA-Protein Crosslinks

DOI:
10.3791/200209-v

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02:49 min

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April 21, 2023

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1Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Tags

DNACrosslinked ProteinsIsolationNormalizationUbiquitylationSUMOylationPBSDNAzolEthanolNucleic AcidCentrifugationPelletDNA ConcentrationUV-Vis SpectrometerMicrococcal NucleaseCalcium Micrococcal Nuclease Reaction Buffer

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