Isolation of Marginating Hepatic Leukocytes from a Mouse Liver

Published: July 31, 2023

Abstract

Source: Sorski, L., et al. Selective Harvesting of Marginating-hepatic Leukocytes. J. Vis. Exp. (2016)

In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Mouse Protocol Perfusion of the Liver and Collection of MH-Leukocytes Euthanize the animal with an overdose of 8% isoflurane. As a precaution, place a 15 ml tube containing an isoflurane-soaked pad around the mouse's head until opening its chest cavity. Fix the limbs of the mo…

開示

The authors have nothing to disclose.

Materials

Autoclud Peristaltic pump
Butterfly needle OMG 26G
Butterfly needle OMG 21G*3/4"
Syringe Pic solution 1 ml
Syringe Pic solution 2.5 ml
Syringe Pic solution 5 ml
Syringe Pic solution 10 ml
Syringe Pic solution 25 ml
Blunted-edged forceps
Scissors
Hemostat
Tissue forceps

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記事を引用
Isolation of Marginating Hepatic Leukocytes from a Mouse Liver. J. Vis. Exp. (Pending Publication), e21531, doi: (2023).

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