Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
Published: July 31, 2023
Abstract
Source: Sorski, L., et al. Selective Harvesting of Marginating-hepatic Leukocytes. J. Vis. Exp. (2016)
In this video, we demonstrate the isolation of marginating hepatic leukocytes from mouse liver by forced perfusion.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Mouse Protocol Perfusion of the Liver and Collection of MH-Leukocytes Euthanize the animal with an overdose of 8% isoflurane. As a precaution, place a 15 ml tube containing an isoflurane-soaked pad around the mouse's head until opening its chest cavity. Fix the limbs of the mo…
開示
The authors have nothing to disclose.
Materials
| Autoclud Peristaltic pump | |||
| Butterfly needle | OMG | 26G | |
| Butterfly needle | OMG | 21G*3/4" | |
| Syringe | Pic solution | 1 ml | |
| Syringe | Pic solution | 2.5 ml | |
| Syringe | Pic solution | 5 ml | |
| Syringe | Pic solution | 10 ml | |
| Syringe | Pic solution | 25 ml | |
| Blunted-edged forceps | |||
| Scissors | |||
| Hemostat | |||
| Tissue forceps |
