Small Molecule Nematicides Screening Assay: A Medium Throughput Method to Screen Potential Nematicides Against Ditylenchus dipsaci

Published: April 30, 2023

Abstract

Source: Cammalleri, S. R., et al. Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci. J. Vis. Exp. (2022).

In this video, we demonstrate a screening assay to identify the efficacy of small-molecule nematicides against nematodes. Nematicides are considered effective if they cause a decrease in the number of mobile nematodes in the sample after exposure.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board 1. In vitro small molecule screen Prepare the assay plates following the steps below. Pour autoclaved distilled water into a sterile trough and, using a multichannel pipette, dispense 40 µL of distilled water from the trough into each well of a flat-bottom 96-well plate. Pre…

開示

The authors have nothing to disclose.

Materials

50mL beaker Pyrex CLS100050
96 well plate Sarstedt 83.3924
aluminium foil Alcan Plus
dissecting scope Leica Leica MZ75
DMSO Sigma-Aldrich 472301-500ML
glass slide MAGNA 60-1200
Lint-Free Blotting Paper V&P Scientific VP 522-100
Low retention pipette tips (200uL) LABFORCE 1159M44
multichannel pipette Eppendorf research plus 3125000036
NaOH Sigma-Aldrich S8045-500G
parafilm Bemis PM-996
pin cleaning solutions V&P Scientific VP110A
pinner V&P Scientific VP381N
pinner rinse trays V&P Scientific VP 421
reagent reservoir with lid for multichannel pipettes Sigma-Aldrich BR703459
shaking incubator New Brunswick Scientific I26

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記事を引用
Small Molecule Nematicides Screening Assay: A Medium Throughput Method to Screen Potential Nematicides Against Ditylenchus dipsaci . J. Vis. Exp. (Pending Publication), e21166, doi: (2023).

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