Source: Cammalleri, S. R., et al. Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci. J. Vis. Exp. (2022).
In this video, we demonstrate a screening assay to identify the efficacy of small-molecule nematicides against nematodes. Nematicides are considered effective if they cause a decrease in the number of mobile nematodes in the sample after exposure.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board
1. In vitro small molecule screen
2. Data collection and analysis
The authors have nothing to disclose.
50mL beaker | Pyrex | CLS100050 | |
96 well plate | Sarstedt | 83.3924 | |
aluminium foil | Alcan Plus | ||
dissecting scope | Leica | Leica MZ75 | |
DMSO | Sigma-Aldrich | 472301-500ML | |
glass slide | MAGNA | 60-1200 | |
Lint-Free Blotting Paper | V&P Scientific | VP 522-100 | |
Low retention pipette tips (200uL) | LABFORCE | 1159M44 | |
multichannel pipette | Eppendorf research plus | 3125000036 | |
NaOH | Sigma-Aldrich | S8045-500G | |
parafilm | Bemis | PM-996 | |
pin cleaning solutions | V&P Scientific | VP110A | |
pinner | V&P Scientific | VP381N | |
pinner rinse trays | V&P Scientific | VP 421 | |
reagent reservoir with lid for multichannel pipettes | Sigma-Aldrich | BR703459 | |
shaking incubator | New Brunswick Scientific | I26 |