Source: Ellis, J. L et al., Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish. J. Vis. Exp. (2017).
In this video, we describe a technique of hematoxylin and eosin staining of paraffin-embedded tissue sections to study their histology. The procedure includes deparaffinization of the tissue followed by staining the cells in the tissue. Upon staining, the nucleus appears distinctly blue, and the proteins in the cytoplasm display pink coloration.
1. Preparation of staining solutions
2. Hematoxylin and Eosin Staining of Paraffin Sections
3. Imaging and Storage of Stained Slides
The authors have nothing to disclose.
95% ethanol (EtOH) | Decon Labs, Inc. | 2801 | Flammable |
Charged slides | Fisher Scientific | 12-550-16 | |
Dissecting microscope | Leica Biosystems | Leica Mz 95 | |
Eosin-Phloxine stain set | Newcomer Supply | 1082A | |
Ethyl alcohol | Sigma-Aldrich | E7023 | Flammable |
Harris hematoxylin | Poly Scientific R&D Corp. | s212 | Irritant |
Hydrochloric acid (HCl) | Fisher Scientific | A144 | Irritant |
Isopropanol | Newcomer Supply | 12094E | Flammable |
Xylenes | Fisher Scientific | X3S-4 | Irritant |