H&E Staining of Paraffin-Embedded Tissue Sections: A Differential Staining Technique to Visualize Liver Tissue Sections Using Combination of Hematoxylin and Eosin Dyes
Published: April 30, 2023
Abstract
Source: Ellis, J. L et al., Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish. J. Vis. Exp. (2017).
In this video, we describe a technique of hematoxylin and eosin staining of paraffin-embedded tissue sections to study their histology. The procedure includes deparaffinization of the tissue followed by staining the cells in the tissue. Upon staining, the nucleus appears distinctly blue, and the proteins in the cytoplasm display pink coloration.
Protocol
1. Preparation of staining solutions Filter Harris hematoxylin stock solution to remove any solids that have precipitated. Fold a coffee filter in half so that the filter creates a cone. Open the side panel so that the liquid runs through the filter, allowing any debris to be caught. Place the filter inside a funnel and the funnel into a glass media bottle. Gradually pour the stock hematoxylin through the filter. CAUTION: Hematoxylin is hazardous in…
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The authors have nothing to disclose.
Materials
| 95% ethanol (EtOH) | Decon Labs, Inc. | 2801 | Flammable |
| Charged slides | Fisher Scientific | 12-550-16 | |
| Dissecting microscope | Leica Biosystems | Leica Mz 95 | |
| Eosin-Phloxine stain set | Newcomer Supply | 1082A | |
| Ethyl alcohol | Sigma-Aldrich | E7023 | Flammable |
| Harris hematoxylin | Poly Scientific R&D Corp. | s212 | Irritant |
| Hydrochloric acid (HCl) | Fisher Scientific | A144 | Irritant |
| Isopropanol | Newcomer Supply | 12094E | Flammable |
| Xylenes | Fisher Scientific | X3S-4 | Irritant |
