JOVE JOURNAL

遺伝学

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Huan-Huan Wei*¹, Yuanlong Liu*¹, Yang Wang², Qianyun Lu¹, Xuerong Yang¹, Jiefu Li¹, Zefeng Wang¹

¹Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology,Shanghai Institutes for Biological Sciences (SIBS), ²Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center,Dalian Medical University

Journal (Genetics)

Single Molecule Analysis of Laser Localized Psoralen Adducts

Jing Huang¹, Himabindu Gali¹, Julia Gichimu¹, Marina A. Bellani¹, Durga Pokharel¹, Manikandan Paramasivam¹, Michael M. Seidman¹

¹Laboratory of Molecular Gerontology,National Institute on Aging, National Institutes of Health

Journal (Genetics)

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Muhammad Khairul Ramlee¹, Jing Wang¹, Alice M. S. Cheung¹, Shang Li¹

¹Cancer & Stem Cell Biology Programme,Duke-NUS Medical School

Journal (Genetics)

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Francis R. G. Amrit¹, Arjumand Ghazi¹

¹小児科,University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh

Journal (Genetics)

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Joanna M. Feehan*^1,2, Katherine E. Scheibel*¹, Salim Bourras³, William Underwood⁴, Beat Keller³, Shauna C. Somerville¹

¹Department of Plant and Microbial Biology,University of California Berkeley, ²John Innes Centre,Norwich Research Park, ³Department of Plant and Microbial Biology,University of Zürich, ⁴USDA-ARS Sunflower and Plant Biology Research Unit

Journal (Genetics)

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

Adam Z. Albitar¹, Wanlong Ma¹, Maher Albitar¹

¹NeoGenomics Laboratories

Journal (Genetics)

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Zuzer Dhoondia¹, Ricci Tarockoff¹, Nadra Alhusini¹, Scott Medler¹, Neha Agarwal¹, Athar Ansari¹

¹Department of Biological Sciences,Wayne State University

Journal (Genetics)

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Zhong-Dong Shi*¹, Chew-Li Soh*¹, Zengrong Zhu*¹, Danwei Huangfu¹

¹Developmental Biology Program,Sloan Kettering Institute

Journal (Genetics)

Generation of Fluorescent Protein Fusions in Candida Species

Sara Gonia¹, Judith Berman², Cheryl A. Gale¹

¹小児科,University of Minnesota, ²Department of Molecular Microbiology and Biotechnology,テルアビブ大学

Journal (Genetics)

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Alfredo J. Hernandez¹, Charles C. Richardson¹

¹Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Journal (Genetics)

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Joshua R. Porter¹, William G. Telford², Eric Batchelor¹

¹Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, ²Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

Journal (Genetics)

Controllable Ion Channel Expression through Inducible Transient Transfection

Matan Geron*¹, Adina Hazan*¹, Avi Priel¹

¹The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine,The Hebrew University of Jerusalem (HUJI)

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