JOVE JOURNAL

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Journal (Cancer Research)

Using the Dot Assay to Analyze Migration of Cell Sheets

Christina H. Stuelten¹

¹Laboratory of Cellular and Molecular Biology, National Cancer Institute,National Institutes of Health (NIH)

Journal (Cancer Research)

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Marta H. Kubala^1,2, Yves A. DeClerck^1,2,3

¹Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics,University of Southern California, ²The Saban Research Institute of Children's Hospital Los Angeles, ³生化学・分子医学科,University of Southern California

Journal (Cancer Research)

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

Jean-Baptiste Guy*^1,2, Sophie Espenel*^1,2, Alexis Vallard^1,2, Priscillia Battiston-Montagne¹, Anne-Sophie Wozny^1,3, Dominique Ardail^1,3, Gersende Alphonse^1,3, Chloé Rancoule^1,2, Claire Rodriguez-Lafrasse^1,3, Nicolas Magne^1,2

¹UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud,Université Lyon 1, ²Département de Radiothérapie,Institut de Cancérologie de la Loire - Lucien Neuwirth, ³Hospices Civils de Lyon,Centre Hospitalier Lyon-Sud

Journal (Cancer Research)

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Magdalena Delgado*¹, Anisha Kothari*¹, Walter N. Hittelman², Timothy C. Chambers¹

¹生化学・分子生物学科,University of Arkansas for Medical Sciences, ²Department of Experimental Therapeutics,University of Texas MD Anderson Cancer Center

Journal (Cancer Research)

Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Henning D. Popp¹, Susanne Brendel¹, Wolf-Karsten Hofmann¹, Alice Fabarius¹

¹Department of Hematology and Oncology,Medical Faculty Mannheim of the Heidelberg University

Journal (Cancer Research)

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier (MSC) for Lung Cancer Screening

Mavis Mensah*¹, Cristina Borzi*¹, Carla Verri¹, Paola Suatoni², Davide Conte¹, Ugo Pastorino², Fortunato Orazio¹, Gabriella Sozzi¹, Mattia Boeri¹

¹Department of Experimental Oncology and Molecular Medicine,Fondazione IRCCS Istituto Nazionale dei Tumori, ²Unit of Thoracic Surgery,Fondazione IRCCS Istituto Nazionale dei Tumori

Journal (Cancer Research)

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy

Daijiro Kobayashi¹, Atsushi Shibata², Takahiro Oike¹, Takashi Nakano¹

¹放射線腫瘍学科,Gunma University Graduate School of Medicine, ²Advanced Scientific Research Leaders Development Unit,Gunma University Graduate School of Medicine

Journal (Cancer Research)

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

George Zhang¹, Yudan Chi¹, Yi-Chieh Nancy Du¹

¹Department of Pathology and Laboratory Medicine,Weill Cornell Medicine

Journal (Cancer Research)

Evaluating In Vitro DNA Damage Using Comet Assay

Yanxin Lu^1,2, Yang Liu¹, Chunzhang Yang¹

¹Neuro-Oncology Branch, Center for Cancer Research,National Cancer Institute, ²Basic Medical Science Department,Zunyi Medical College-Zhuhai Campus

Journal (Cancer Research)

Sample Extraction and Simultaneous Chromatographic Quantitation of Doxorubicin and Mitomycin C Following Drug Combination Delivery in Nanoparticles to Tumor-bearing Mice

Rui Xue Zhang¹, Tian Zhang¹, King Chen¹, Ji Cheng¹, Paris Lai¹, Andrew M. Rauth², K. Sandy Pang¹, Xiao Yu Wu¹

¹Department of Pharmaceutical Sciences,University of Toronto, ²Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario Cancer Institute,University Health Network

Journal (Cancer Research)

A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

Erik S. Carlson^1,2, Pramod Upadhyaya², Stephen S. Hecht²

¹Department of Pharmacology,University of Minnesota, ²Masonic Cancer Center,University of Minnesota

Journal (Cancer Research)

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

Laura Mercatali¹, Chiara Spadazzi¹, Giacomo Miserocchi¹, Chiara Liverani¹, Alessandro De Vita¹, Alberto Bongiovanni¹, Federica Recine¹, Dino Amadori¹, Toni Ibrahim¹

¹Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST),IRCCS

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