JOVE JOURNAL

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Showing 1 - 12 in 611 videos.

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing

Jacy Fang*^1,2, Isabella Salinas*^1,2, Sarah San Vicente*^1,2, Caroline Zielinski*^1,2, Moshe Sade-Feldman^1,2,3

¹Krantz Family Center for Cancer Research, Department of Medicine,Massachusetts General Hospital, ²Broad Institute of MIT and Harvard, ³医学部,Harvard Medical School

Journal (Cancer Research)

Rapid Dissociation of Tumor Tissue to Gain Enhanced Single-Cell Tumor Insights

Jacy Fang*^1,2, Isabella Salinas*^1,2, Sarah San Vicente*^1,2, Caroline Zielinski*^1,2, Moshe Sade-Feldman^1,2,3

¹Krantz Family Center for Cancer Research, Department of Medicine,Massachusetts General Hospital, ²Broad Institute of MIT and Harvard, ³医学部,Harvard Medical School

Journal (Cancer Research)

Red Blood Cell Lysis in Digested Tumor Samples for Single-Cell RNA Sequencing

Jacy Fang*^1,2, Isabella Salinas*^1,2, Sarah San Vicente*^1,2, Caroline Zielinski*^1,2, Moshe Sade-Feldman^1,2,3

¹Krantz Family Center for Cancer Research, Department of Medicine,Massachusetts General Hospital, ²Broad Institute of MIT and Harvard, ³医学部,Harvard Medical School

Journal (Cancer Research)

Dead Cell Removal from Dissociated Tumor for Single-Cell Sequencing

Jacy Fang*^1,2, Isabella Salinas*^1,2, Sarah San Vicente*^1,2, Caroline Zielinski*^1,2, Moshe Sade-Feldman^1,2,3

¹Krantz Family Center for Cancer Research, Department of Medicine,Massachusetts General Hospital, ²Broad Institute of MIT and Harvard, ³医学部,Harvard Medical School

Journal (Cancer Research)

Optimization and In Vivo Propagation of CellTrace Far Red (CT-FR) Stained Zebrafish T-ALL Cells

Majd A. Al-Hamaly^1,2, Yelena Chernyavskaya³, Jessica S. Blackburn^2,3

¹Pharmacology and Nutritional Sciences,The University of Kentucky, ²Markey Cancer Center,The University of Kentucky, ³Department of Molecular and Cellular Biochemistry,The University of Kentucky

Journal (Cancer Research)

Preparation and Sorting of CT-FR-Stained Zebrafish T-ALL Cells for Transplantation and Tumor Tracking

Majd A. Al-Hamaly^1,2, Yelena Chernyavskaya³, Jessica S. Blackburn^2,3

¹Pharmacology and Nutritional Sciences,The University of Kentucky, ²Markey Cancer Center,The University of Kentucky, ³Department of Molecular and Cellular Biochemistry,The University of Kentucky

Journal (Cancer Research)

Identification of Quiescent Cells in a Zebrafish T-Cell Acute Lymphoblastic Leukemia Model Using Cell Proliferation Staining

Majd A. Al-Hamaly^1,2, Yelena Chernyavskaya³, Jessica S. Blackburn^2,3

¹Pharmacology and Nutritional Sciences,The University of Kentucky, ²Markey Cancer Center,The University of Kentucky, ³Department of Molecular and Cellular Biochemistry,The University of Kentucky

Journal (Cancer Research)

In Vivo Propagation and Harvesting of GFP-Labeled Zebrafish T-ALL Cells

Majd A. Al-Hamaly^1,2, Yelena Chernyavskaya³, Jessica S. Blackburn^2,3

¹Pharmacology and Nutritional Sciences,The University of Kentucky, ²Markey Cancer Center,The University of Kentucky, ³Department of Molecular and Cellular Biochemistry,The University of Kentucky

Journal (Cancer Research)

Injecting Labeled Leukemia Cells into Zebrafish Embryos for Assessing Tumor Behavior

Monika Verma¹, Michele Rhodes¹, Susan Shinton¹, David L. Wiest¹

¹Nuclear Dynamics and Cancer Program,Fox Chase Cancer Center

Journal (Cancer Research)

Preparation of Single-Cell Embryo Suspension for Flow Cytometry Analysis to Assess Tumor Xenotransplantation

Monika Verma¹, Michele Rhodes¹, Susan Shinton¹, David L. Wiest¹

¹Nuclear Dynamics and Cancer Program,Fox Chase Cancer Center

Journal (Cancer Research)

A Simple, Rapid, and Effective Method for Tumor Xenotransplantation Analysis in Transparent Zebrafish Embryos

Monika Verma¹, Michele Rhodes¹, Susan Shinton¹, David L. Wiest¹

¹Nuclear Dynamics and Cancer Program,Fox Chase Cancer Center

Journal (Cancer Research)

Transient Transfection of Extrachromosomal Reporter Substrates and Luminescence Detection to Study DNA Double Strand Break Repair Pathways

Diego Grande*¹, Eeson Rajendra*¹, Bethany Mason¹, Alessandro Galbiati¹, Simon J. Boulton^1,2, Graeme C. M. Smith¹, Helen M. R. Robinson¹

¹Artios Pharma Ltd, Cambridge, ²The Francis Crick Institute, London

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