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Journal (Cancer Research)

RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells

Junli Chang*^1,2, Xiaoping Ma*^1,2, Xingyuan Sun^1,2, Chujie Zhou^1,2, Peng Zhao^1,2, Yongjun Wang^1,2, Yanping Yang^1,2

¹Longhua Hospital,Shanghai University of Traditional Chinese Medicine, ²Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education

Journal (Cancer Research)

Screening Ion Channels in Cancer Cells

Laura Kallay¹, Vaibhavkumar S. Gawali¹, Donatien Kamdem Toukam¹, Debanjan Bhattacharya¹, Andrew Jenkins², Soma Sengupta³, Daniel A. Pomeranz Krummel³

¹Department of Neurology and Rehabilitation Medicine, Division of Neuro-Oncology,University of Cincinnati College of Medicine, ²Department of Pharmaceutical Sciences, School of Pharmacy,University of Saint Joseph, ³The Vontz Center for Molecular Studies,University of Cincinnati College of Medicine

Journal (Cancer Research)

Testing the Polarized State of Mitochondria to Analyze the Ion Channel Function in Cancer Cells

Laura Kallay¹, Vaibhavkumar S. Gawali¹, Donatien Kamdem Toukam¹, Debanjan Bhattacharya¹, Andrew Jenkins², Soma Sengupta³, Daniel A. Pomeranz Krummel³

¹Department of Neurology and Rehabilitation Medicine, Division of Neuro-Oncology,University of Cincinnati College of Medicine, ²Department of Pharmaceutical Sciences, School of Pharmacy,University of Saint Joseph, ³The Vontz Center for Molecular Studies,University of Cincinnati College of Medicine

Journal (Cancer Research)

In Vitro Drug Validation to Assay Ion Channel Function in Cancer Cells

Laura Kallay¹, Vaibhavkumar S. Gawali¹, Donatien Kamdem Toukam¹, Debanjan Bhattacharya¹, Andrew Jenkins², Soma Sengupta³, Daniel A. Pomeranz Krummel³

¹Department of Neurology and Rehabilitation Medicine, Division of Neuro-Oncology,University of Cincinnati College of Medicine, ²Department of Pharmaceutical Sciences, School of Pharmacy,University of Saint Joseph, ³The Vontz Center for Molecular Studies,University of Cincinnati College of Medicine

Journal (Cancer Research)

Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia

Gisele O. L. Rodrigues¹, WenQing Li¹, Sarah D. Cramer^1,2,3, Hila Y. Winer¹, Tu Chun Hsu^1,2,3, Timothy Gower⁴, Julie A. Hixon¹, Scott K. Durum¹

¹Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute,National Institutes of Health (NIH), ²Comparative Biomedical Scientist Training Program,NIH, ³Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory,Michigan State University, ⁴NCI-Frederick Laboratory Animal Sciences Program

Journal (Cancer Research)

Establishment and Characterization of Patient-Derived Xenograft Models of Anaplastic Thyroid Carcinoma and Head and Neck Squamous Cell Carcinoma

Maodi Wu^1,3, Yang Liu², Yi Zhao³, Yuyao Zhang⁴, Liansi Huang³, Qijun Du³, Ting Zhang^1,3, Zhihui Zhong^1,3, Han Luo², Kai Xiao^1,3

¹Precision Medicine Research Center, Sichuan Provincial Key Laboratory of Precision Medicine, National Clinical Research Center for Geriatrics, West China Hospital,Sichuan University, ²Department of Thyroid Surgery, West China Hospital,Sichuan University, ³Sichuan Kangcheng Biotech Co., ⁴Sichuan Cancer Hospital

Journal (Cancer Research)

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Nicolas Huyghe¹, Elena Benidovskaya¹, Simon Beyaert¹, Aurélie Daumerie⁴, Finoula Maestre Osorio¹, Frank Aboubakar Nana^2,3, Caroline Bouzin⁴, Marc Van den Eynde^1,5

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

Journal (Cancer Research)

Multiplex Immunofluorescence Staining and Image Analysis

Nicolas Huyghe¹, Elena Benidovskaya¹, Simon Beyaert¹, Aurélie Daumerie⁴, Finoula Maestre Osorio¹, Frank Aboubakar Nana^2,3, Caroline Bouzin⁴, Marc Van den Eynde^1,5

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

Journal (Cancer Research)

Pancreatic Tissue Dissection to Isolate Viable Single Cells

Oshri Yosefov-Levi¹, Sharona Tornovsky¹, Oren Parnas¹

¹The Lautenberg Center for Immunology and Cancer Research, The Institute for Medical Research Israel-Canada, Faculty of Medicine,The Hebrew University of Jerusalem

Journal (Cancer Research)

Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3,Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678),Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine,University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology,University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program,Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute,Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit,Hospital Universitario Ramón y Cajal, ¹⁰病理学科,Hospital Universitario Ramón y Cajal, ¹¹放射線腫瘍学科,Hospital Universitario Ramón y Cajal, ¹²Department of Cancer,Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3,IRYCIS

Journal (Cancer Research)

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3,Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678),Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine,University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology,University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program,Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute,Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit,Hospital Universitario Ramón y Cajal, ¹⁰病理学科,Hospital Universitario Ramón y Cajal, ¹¹放射線腫瘍学科,Hospital Universitario Ramón y Cajal, ¹²Department of Cancer,Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3,IRYCIS

Journal (Cancer Research)

Microfluidic Shear Apparatus Setup for Single-Cell Fluid Shear Assay

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera¹, Maksym Zarodniuk¹, John D. Obayemi², Winston O. Soboyejo², Meenal Datta¹

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

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