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Double immunofluorescence de γH2AX et 53BP1 dans les lymphocytes périphériques humains
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Genetica
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JoVE Journal Genetica
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
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Double immunofluorescence de γH2AX et 53BP1 dans les lymphocytes périphériques humains

1,052 Views

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05:34 min

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July 14, 2023

DOI:

10.3791/65472-v

DOI:
10.3791/65472-v

•

05:34 min
July 14, 2023

•

7 Views
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1Unità di Genetica, Dipartimento di Biologia,University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology,Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico

Summary

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Ce protocole présente une méthode pour évaluer la formation et la réparation des cassures double brin d’ADN par la détection simultanée des foyers γH2AX et 53BP1 dans les noyaux interphasiques des lymphocytes périphériques humains traités à la bléomycine.

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Cite this Article

Falaschi, A., Chiaramonte, A., Testi, S., Scarpato, R. Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes. J. Vis. Exp. (197), e65472, doi:10.3791/65472 (2023).

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