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人外周淋巴细胞中γH2AX和53BP1的双重免疫荧光
JoVE Journal
Genetica
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JoVE Journal Genetica
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
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人外周淋巴细胞中γH2AX和53BP1的双重免疫荧光

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05:34 min

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July 14, 2023

DOI:

10.3791/65472-v

DOI:
10.3791/65472-v

•

05:34 min
July 14, 2023

•

7 Views
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1Unità di Genetica, Dipartimento di Biologia,University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology,Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico

Summary

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该协议提出了一种通过同时检测博来霉素处理的人外周淋巴细胞的间期核中的γH 2AX和53BP1病灶来评估DNA双链断裂的形成和修复的方法。

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Cite this Article

Falaschi, A., Chiaramonte, A., Testi, S., Scarpato, R. Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes. J. Vis. Exp. (197), e65472, doi:10.3791/65472 (2023).

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