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Isolement de vésicules extracellulaires à faible teneur en endotoxines dérivées de lignées cellulaires cancéreuses
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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Isolement de vésicules extracellulaires à faible teneur en endotoxines dérivées de lignées cellulaires cancéreuses

789 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

•

4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Summary

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Le protocole proposé comprend des lignes directrices sur la façon d’éviter la contamination par l’endotoxine lors de l’isolement des vésicules extracellulaires à partir de surnageants de culture cellulaire, et sur la façon de les évaluer correctement.

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Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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