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Visualization of Organelles In Situ by Cryo-STEM Tomography
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Biologia
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JoVE Journal Biologia
Visualization of Organelles In Situ by Cryo-STEM Tomography

Visualization of Organelles In Situ by Cryo-STEM Tomography

DOI:
10.3791/65052-v

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08:37 min

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June 23, 2023

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, , ,
1Department of Chemical and Biological Physics,Weizmann Institute of Sciences, 2Department of Chemical Research Support,Weizmann Institute of Sciences

Capitoli

  • 00:05Introduction
  • 00:48STEM Setup
  • 04:37Placing the Specimen and Recording an Image
  • 06:58Recording an Image
  • 07:23Results: Visualization of the Organelles
  • 08:03Conclusion

Summary

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Cryo-STEM tomography provides a means to visualize organelles of intact cells without embedding, sectioning, or other invasive preparations. The 3D resolution obtained is currently in the range of a few nanometers, with a field of view of several micrometers and an accessible thickness in the order of 1 µm.

Tags

Keywords: Cryo-STEM TomographyTEM TomographyFIB SEMSoft X-ray TomographySTEM OpticsColumn AlignmentEucentric FocusSTEM ModeCondenser ApertureBeam ShiftRotation CenterPivot PointsCondenser Stigmators

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