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Visualization of Organelles In Situ by Cryo-STEM Tomography
JoVE 杂志
生物学
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JoVE 杂志 生物学
Visualization of Organelles In Situ by Cryo-STEM Tomography

Visualization of Organelles In Situ by Cryo-STEM Tomography

1,499 Views

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08:37 min

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June 23, 2023

DOI:

10.3791/65052-v

DOI:
10.3791/65052-v

•

08:37 min
June 23, 2023

•

9 Views
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1Department of Chemical and Biological Physics,Weizmann Institute of Sciences, 2Department of Chemical Research Support,Weizmann Institute of Sciences

Summary

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Cryo-STEM tomography provides a means to visualize organelles of intact cells without embedding, sectioning, or other invasive preparations. The 3D resolution obtained is currently in the range of a few nanometers, with a field of view of several micrometers and an accessible thickness in the order of 1 µm.

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Cite this Article

Kirchweger, P., Mullick, D., Wolf, S. G., Elbaum, M. Visualization of Organelles In Situ by Cryo-STEM Tomography. J. Vis. Exp. (196), e65052, doi:10.3791/65052 (2023).

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