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シングルセルマルチオミクスのための瞬間凍結肝臓組織からの核の単離
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
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シングルセルマルチオミクスのための瞬間凍結肝臓組織からの核の単離

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09:09 min

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December 09, 2022

DOI:

10.3791/64792-v

DOI:
10.3791/64792-v

•

09:09 min
December 09, 2022

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75 Views
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1Helmholtz Pioneer Campus (HPC),Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department,Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine,Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine,Technical University of Munich

Summary

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ここでは、一核RNA-seq、ATAC-seq、およびジョイントマルチオミクス(RNA-seqおよびATAC-seq)について、瞬間凍結されたアーカイブされた肝臓組織から核を単離するためのプロトコルを紹介します。

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Cite this Article

Strzelecki, M., Yin, K., Talavera-López, C., Martinez-Jimenez, C. P. Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics. J. Vis. Exp. (190), e64792, doi:10.3791/64792 (2022).

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