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大鼠背根神经节外植体和雪旺细胞共培养中外周轴突的体外髓鞘形成

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

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大鼠背根神经节外植体和雪旺细胞共培养中外周轴突的体外髓鞘形成

DOI:

10.3791/64768-v

•

08:57 min

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February 10, 2023

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Alina Blusch, Melissa Sgodzai, Niklas Rilke, Jeremias Motte, Jennifer König, Kalliopi Pitarokoili, Thomas Grüter

¹Department of Neurology,Ruhr-University Bochum, St. Josef Hospital

Capitoli

00:05Introduction
00:49Sciatic Nerve Preparation and Refurbishment
02:18Enzymatic Digestion of the Sciatic Nerve
03:04Cell Separation
03:59Schwann Cell Magnetic Separation
04:56Dorsal Root Ganglia (DRG) Preparation
06:04DRG Cell Culture and Coculture with Schwann Cells
07:13Results: Myelination in Coculture of DRG Explants and Schwann Cells
08:17Conclusion

Summary

Traduzione automatica

在背根神经节和雪旺细胞的共培养系统中，可以研究周围神经系统的髓鞘形成。该模型为观察和量化外周髓鞘形成以及研究目标化合物对髓鞘的影响提供了实验机会。

Tags

Keywords: In Vitro Myelination Peripheral Axons Dorsal Root Ganglion Explants Schwann Cells Peripheral Nervous System Axon-Schwann Cell Interactions Myelination Quantification DRG Explant Cultures Neurodegenerative Diseases Peripheral Nerve System Sciatic Nerve Nerve Fibers Enzymatic Digestion

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