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使用扩增显微镜物理放大 整个果蝇 胚胎以进行超分辨率成像
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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使用扩增显微镜物理放大 整个果蝇 胚胎以进行超分辨率成像

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09:11 min

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April 28, 2023

DOI:

10.3791/64662-v

DOI:
10.3791/64662-v

•

09:11 min
April 28, 2023

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1 Views
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1Department of Biological Sciences,University of Arkansas

Tags

Expansion MicroscopyWhole-mount Drosophila EmbryosSuper-resolution ImagingProtein LocalizationCell MorphologyAgar SlabDouble-sided TapeVitelline MembranePoly-L-LysinePBS Tween

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Parveen, S., Jones, N. W., Millerschultz, I., Paré, A. C. Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging. J. Vis. Exp. (194), e64662, doi:10.3791/64662 (2023).

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