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从人类扩增的潜在干细胞中产生自然杀伤细胞
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells
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从人类扩增的潜在干细胞中产生自然杀伤细胞

2,379 Views

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06:53 min

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January 13, 2023

DOI:

10.3791/64608-v

DOI:
10.3791/64608-v

•

06:53 min
January 13, 2023

•

11 Views
, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

Summary

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本协议显示了如何在3D和2D培养条件下区分具有轻度细胞毒性的CD3 - / CD45 + CD56 +细胞与人扩增潜在干细胞（hEPSC）。这允许在不破坏复杂微环境的情况下进行常规表型验证。

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Cite this Article

Yu Ching, P., Wang, C., Hang, S., Liu, P., Sugimura, R. Generation of Natural Killer Cells from Human Expanded Potential Stem Cells. J. Vis. Exp. (191), e64608, doi:10.3791/64608 (2023).

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