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从P3HR1细胞系分离和定量EB病毒
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
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从P3HR1细胞系分离和定量EB病毒

DOI:
10.3791/64279-v

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09:14 min

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September 28, 2022

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, ,
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine,American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine,American University of Beirut

Capitoli

  • 00:05Introduction
  • 00:36Preparing the Plate for Culture
  • 01:49Induction and Isolation of Epstein-Barr Virus Particles
  • 02:45DNA Extraction from Viral Particles
  • 04:45Checking DNA Concentration and Purity
  • 05:34Quantification by Real-Time Polymerase Chain Reaction
  • 07:54Results: Cell Counting and Induction of P3HR-1 Cells with PMA and DMSO
  • 08:48Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议允许在用佛波醇12-肉豆蔻酸酯13-乙酸酯诱导病毒裂解循环时从人P3HR1细胞系中分离Epstein-Barr病毒颗粒。随后从病毒制剂中提取DNA，并进行实时PCR以量化病毒颗粒浓度。

Tags

Epstein-Barr VirusP3HR1 Cell LineViral StockVirus IsolationVirus QuantificationPMADMSOCell CultureCentrifugationPhenol-chloroform ExtractionEthanol Precipitation

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