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Rivista
/
Neuroscienze
/
使用双光子全息显微镜评估和操纵神经活动
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal
Neuroscienze
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用双光子全息显微镜评估和操纵神经活动
DOI:
10.3791/64205-v
•
10:09 min
•
September 16, 2022
•
Daisuke Kato
2
,
Xiangyu Quan
,
Yuta Tanisumi
2
,
Zhongtian Guo
2
,
Mitsuhiro Morita
,
Tetsuya Takiguchi
,
Osamu Matoba
,
Hiroaki Wake
2,6
1
Department of Anatomy and Molecular Cell Biology
,
Nagoya University Graduate School of Medicine
,
2
Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences
,
National Institutes of Natural Sciences
,
3
Department of System Science
,
Kobe University Graduate School of System Informatics
,
4
Department of Biology, Graduate School of Sciences
,
Kobe University
,
5
Department of Information Science
,
Kobe University Graduate School of System Informatics
,
6
Center of Optical Scattering Image Science
,
Kobe University
Capitoli
00:04
Introduction
00:55
Surgery and Adeno‐Associated Virus (AAV) Injection
02:50
Preparation for the Holographic Stimulation or Illumination System
04:54
Ca
2+
Imaging Using an Image Sensor with Holographic Illumination
06:06
Two‐Photon Imaging with Optogenetics Using a Holographic Microscope
08:04
Results: Imaging and Functional Connectivity Using a Holographic Microscope
09:26
Conclusion
Summary
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我们开发了一种双光子全息显微镜,可以使用高时空分辨率可视化、评估和操纵神经活动,目的是阐明与异常神经活动相关的神经精神疾病的发病机制。
Tags
Two-photon Holographic Microscopy
Neuroactivity
Neural Activity Manipulation
Spatial Temporal Resolution
Neuropsychiatric Disorders
AAV Injection
Agarose Brain Window
Holographic Stimulation System
Article
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