ジャーナル
/
神経科学
/
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
JoVE Journal
神経科学
This content is Free Access.
JoVE Journal 神経科学
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

DOI:
10.3791/64205-v

•

10:09 min

•

September 16, 2022

•

, , , , , , ,
1Department of Anatomy and Molecular Cell Biology,Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences,National Institutes of Natural Sciences, 3Department of System Science,Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences,Kobe University, 5Department of Information Science,Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science,Kobe University

章

  • 00:04Introduction
  • 00:55Surgery and Adeno‐Associated Virus (AAV) Injection
  • 02:50Preparation for the Holographic Stimulation or Illumination System
  • 04:54Ca2+ Imaging Using an Image Sensor with Holographic Illumination
  • 06:06Two‐Photon Imaging with Optogenetics Using a Holographic Microscope
  • 08:04Results: Imaging and Functional Connectivity Using a Holographic Microscope
  • 09:26Conclusion

概要

自動翻訳

自動翻訳

We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.

タグ

Two-photon Holographic MicroscopyNeuroactivityNeural Activity ManipulationSpatial Temporal ResolutionNeuropsychiatric DisordersAAV InjectionAgarose Brain WindowHolographic Stimulation System

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation

In vivo Imaging of the Mouse Spinal Cord Using Two-photon Microscopy

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

In Vivo Two-Photon Microscopy of Single Nerve Endings in Skin

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Analyzing Neural Activity and Connectivity Using Intracranial EEG Data with SPM Software

In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette

Read Article