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检测胎儿组织来源肠的上皮通透性
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids
DOI:

07:51 min

June 16, 2022

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Capitoli

  • 00:05Introduction
  • 00:53Intestinal Epithelial Cell Plating in Basement Membrane Matrix from Whole Specimens
  • 02:54Immunofluorescent Staining of the Enteroids
  • 03:57Preparation for Microinjection of Enteroids
  • 06:13Results: Visualization of Stained Enteroids
  • 07:19Conclusion

Summary

Traduzione automatica

该协议详细介绍了从胎儿肠道组织建立肠样,一种三维肠道模型。上皮生物标志物的免疫荧光成像用于模型表征。脂多糖(一种细菌内毒素)的顶端暴露,使用显微注射技术以剂量依赖性方式诱导上皮通透性,通过荧光葡聚糖的泄漏来测量。

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