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Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids
JoVE Revista
Medicina
This content is Free Access.
JoVE Revista Medicina
Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

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07:51 min

June 16, 2022

DOI:

07:51 min
June 16, 2022

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Summary

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This protocol details the establishment of enteroids, a three-dimensional intestinal model, from fetal intestinal tissue. Immunofluorescent imaging of epithelial biomarkers was used for model characterization. Apical exposure of lipopolysaccharides, a bacterial endotoxin, using microinjection technique induced epithelial permeability in a dose-dependent manner measured by the leakage of fluorescent dextran.

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