Journal
/
/
Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids
JoVE Revista
Medicina
This content is Free Access.
JoVE Revista Medicina
Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

1,779 Views

07:51 min

June 16, 2022

DOI:

07:51 min
June 16, 2022

5 Views
, , , ,

Summary

Automatically generated

This protocol details the establishment of enteroids, a three-dimensional intestinal model, from fetal intestinal tissue. Immunofluorescent imaging of epithelial biomarkers was used for model characterization. Apical exposure of lipopolysaccharides, a bacterial endotoxin, using microinjection technique induced epithelial permeability in a dose-dependent manner measured by the leakage of fluorescent dextran.

Read Article