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用于测试新兴抗生素的新型高通量 离体 绵羊皮肤伤口模型
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
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用于测试新兴抗生素的新型高通量 离体 绵羊皮肤伤口模型

1,801 Views

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08:30 min

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September 16, 2022

DOI:

10.3791/64041-v

DOI:
10.3791/64041-v

•

08:30 min
September 16, 2022

•

7 Views
, ,
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB),University of Sheffield, 2Department of Chemical and Biological Engineering,University of Sheffield

Summary

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该协议描述了一种逐步建立感染金黄色葡萄球菌的离体绵羊受伤皮肤模型的方法。与传统微生物学技术相比，这种高通量模型更好地模拟体内感染，并为研究人员提供了一个生理相关的平台来测试新兴抗菌剂的功效。

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Ovine Skin Wound ModelHigh-throughput Ex VivoAntimicrobial EfficacyTopical AntimicrobialsWound InfectionsContaminationAseptic TechniqueSkin SampleChlorine Dioxide SolutionSterile PBSAir-liquid Interface

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Cite this Article

Regan, H. C., Taylor, A. F., Karunakaran, E. A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics. J. Vis. Exp. (187), e64041, doi:10.3791/64041 (2022).

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