This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.
Yaozhen, L., Kemin, W., Xiaoyu, J., Yang, O., Hongying, T., Baihong, P. Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis. J. Vis. Exp. (190), e63584, doi:10.3791/63584 (2022).