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Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis
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JoVE 杂志 免疫与感染
Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

Evaluation of a Reliable Biomarker in a Cecal Ligation and Puncture-Induced Mouse Model of Sepsis

DOI:
10.3791/63584-v

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05:28 min

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December 09, 2022

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, , , , ,
1Department of Vascular Surgery, Xiangya Hospital,Central South University

Chapters

  • 00:14Introduction
  • 00:49Preparation
  • 01:15Operation
  • 03:53Treatment
  • 04:52Representative Results
  • 05:16Conclusion

Summary

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This protocol presents the operative details of cecal ligation and puncture (CLP) in a mouse model of sepsis. CLP is one of the most widely used techniques to create an animal model of sepsis. Therefore, a standardized CLP protocol is required for the attainment of reliable research results.

Tags

Cecal Ligation And PunctureCLPSepsisMouse ModelBiomarkerCitrullinated Histone H3CitH3NETosisC57BL/6 MiceAbdominal SurgeryAnesthesiaKetoprofenEuthanasia

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