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Co-cultuur van murine dunne darm epitheliale organoïden met aangeboren lymfoïde cellen
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells
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Co-cultuur van murine dunne darm epitheliale organoïden met aangeboren lymfoïde cellen

4,706 Views

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08:22 min

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March 23, 2022

DOI:

10.3791/63554-v

DOI:
10.3791/63554-v

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08:22 min
March 23, 2022

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14 Views
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1Centre for Host-Microbiome Interactions,King’s College London, 2Wellcome Trust Cell Therapies and Regenerative Medicine Ph.D. Programme,King’s College London, 3Present address: Wellcome Trust/Cancer Research UK Gurdon Institute,Cambridge University

Summary

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Dit protocol biedt gedetailleerde instructies voor het vaststellen van muriene dunne darm organoïden, het isoleren van type-1 aangeboren lymfoïde cellen uit de muriene dunne darm lamina propria, en het vaststellen van 3-dimensionale (3D) co-culturen tussen beide celtypen om bi-directionele interacties tussen intestinale epitheelcellen en type-1 aangeboren lymfoïde cellen te bestuderen.

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Read, E., Jowett, G. M., Coman, D., Neves, J. F. Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells. J. Vis. Exp. (181), e63554, doi:10.3791/63554 (2022).

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