/
/
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
2,361 Views
•
10:46 min
•
February 02, 2022
DOI:
Summary
Automatically generated
The bi-directional mitotic kinesin-5 Cin8 accumulates in clusters that split and merge during their motility. Accumulation in clusters also changes the velocity and directionality of Cin8. Here, a protocol for motility assays with purified Cin8-GFP and analysis of motile properties of single molecules and clusters of Cin8 is described.
Read Article
Cite this Article
Pandey, H., Zvagelsky, T., Popov, M., Sadan, M., Yanir, N., Goldstein-Levitin, A., Siegler, N., Hershfinkel, S., Abraham, Y., Avraham, R., Gheber, L. A., Gheber, L. Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells. J. Vis. Exp. (180), e63425, doi:10.3791/63425 (2022).
Copy