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Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
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Biochimie
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Journal JoVE Biochimie
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

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10:46 min

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February 02, 2022

DOI:

10.3791/63425-v

DOI:
10.3791/63425-v

•

10:46 min
February 02, 2022

•

4 Views
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1Department of Chemistry,Ben-Gurion University of the Negev, Israel, 2Department of Biotechnology Engineering,Ben-Gurion University of the Negev, Israel

Summary

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The bi-directional mitotic kinesin-5 Cin8 accumulates in clusters that split and merge during their motility. Accumulation in clusters also changes the velocity and directionality of Cin8. Here, a protocol for motility assays with purified Cin8-GFP and analysis of motile properties of single molecules and clusters of Cin8 is described.

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Pandey, H., Zvagelsky, T., Popov, M., Sadan, M., Yanir, N., Goldstein-Levitin, A., Siegler, N., Hershfinkel, S., Abraham, Y., Avraham, R., Gheber, L. A., Gheber, L. Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells. J. Vis. Exp. (180), e63425, doi:10.3791/63425 (2022).

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