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通过TIRF显微镜 在体外 同时可视化交联和单微管的动力学
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
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通过TIRF显微镜 在体外 同时可视化交联和单微管的动力学

2,359 Views

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07:20 min

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February 18, 2022

DOI:

10.3791/63377-v

DOI:
10.3791/63377-v

•

07:20 min
February 18, 2022

•

2 Views
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1Biologia Molecolare,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Summary

Automatically generated

在这里，提出了基于TIRF显微镜 的体外 重建测定，以同时量化和比较两个微管群体的动力学。描述了一种同时观察多个微管相关蛋白在交联微管束和单个微管上的集体活性的方法。

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Cite this Article

Mani, N., Marchan, M. F., Subramanian, R. Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy. J. Vis. Exp. (180), e63377, doi:10.3791/63377 (2022).

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