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Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

3,228 Views

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07:44 min

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November 04, 2021

DOI:

10.3791/63168-v

DOI:
10.3791/63168-v

•

07:44 min
November 04, 2021

•

47 Views
, , ,
1Division of Biomedical Sciences, School of Medicine,University of California, Riverside, 2Microscopy and Imaging Core Facility,University of California, Riverside

Summary

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A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.

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Radecki, K. C., Lorenson, M. Y., Carter, D. G., Walker, A. M. Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation. J. Vis. Exp. (177), e63168, doi:10.3791/63168 (2021).

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