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Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
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Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation
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November 04, 2021
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Summary
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A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.
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Radecki, K. C., Lorenson, M. Y., Carter, D. G., Walker, A. M. Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation. J. Vis. Exp. (177), e63168, doi:10.3791/63168 (2021).
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