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果蝇 幼虫注射方案
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JoVE Journal Immunologia e infezioni
Drosophila melanogaster Larva Injection Protocol
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果蝇 幼虫注射方案

DOI:
10.3791/63144-v

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03:16 min

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October 19, 2021

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, ,
1Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences,The George Washington University

Capitoli

  • 00:04Introduction
  • 00:36Larvae Selection for Infection
  • 00:57Injector Preparation
  • 01:28Larvae Injection
  • 02:20Results: Survival and Mortality Rate of Drosophila melanogaster
  • 02:42Conclusion

Summary

Traduzione automatica

Traduzione automatica

果蝇黑色素体 成年蝇已被广泛用作模型生物，以研究宿主抗菌先天免疫反应和微生物感染策略的分子机制。为了促进 D. melanogaster 幼虫阶段作为附加或替代模型系统，描述了幼虫注射技术。

Tags

Drosophila MelanogasterLarvaInjectionProtocolMicroinjectionImmune StudiesMolecular StudiesRinger’s SolutionCapillaryMineral OilNanoliter InjectorTracheal SpiraclesPhotorhabdus AsymbioticaEscherichia ColiInnate ImmunityPathogen VirulenceAlternative Model

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