Journal
/
Immunologie et infection
/
Drosophila melanogaster Larva Injection Protocol
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Drosophila melanogaster Larva Injection Protocol

Drosophila melanogaster Larva Injection Protocol

5,290 Views

•

03:16 min

•

October 19, 2021

DOI:

10.3791/63144-v

DOI:
10.3791/63144-v

•

03:16 min
October 19, 2021

•

41 Views
, ,
1Infection and Innate Immunity Laboratory, Department of Biological Sciences, Institute for Biomedical Sciences,The George Washington University

Summary

Automatically generated

Drosophila melanogaster adult flies have been extensively utilized as model organisms to investigate the molecular mechanisms underlying host antimicrobial innate immune responses and microbial infection strategies. To promote the D. melanogaster larva stage as an additional or alternative model system, a larval injection technique is described.

Explore More Videos

Drosophila MelanogasterLarvaInjectionProtocolMicroinjectionImmune StudiesMolecular StudiesRinger’s SolutionCapillaryMineral OilNanoliter InjectorTracheal SpiraclesPhotorhabdus AsymbioticaEscherichia ColiInnate ImmunityPathogen VirulenceAlternative Model

Read Article

Cite this Article

Tafesh-Edwards, G., Kenney, E., Eleftherianos, I. Drosophila melanogaster Larva Injection Protocol. J. Vis. Exp. (176), e63144, doi:10.3791/63144 (2021).

Download .ris file

Copy

Share Video

.

Copy