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글리코겐 구조 측정을 위한 간 글리코겐 분자 추출
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04:50 min
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February 08, 2022
DOI:
1School of Chemistry and Molecular Biosciences,The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation,The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy,Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy,Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering,Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture,Yangzhou University, 7Glycation and Diabetes Group,Mater Research Institute-The University of Queensland, Translational Research Institute
Summary
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최적의 슈크로스 농도는 슈크로스 밀도 구배 원심분리를 사용하여 간 글리코겐의 추출을 위해 결정하였다. 글리코겐 분해 효소를 억제하기 위해 10분 끓는 단계를 추가하면 유익한 것으로 입증되었습니다.
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Cite this Article
Wang, Z., Liu, Q., Wang, L., Gilbert, R. G., Sullivan, M. A. The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination. J. Vis. Exp. (180), e63088, doi:10.3791/63088 (2022).
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