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뮤린 망막에서 AAV 변환 효율의 정량화를위한 디지털 물방울 PCR 방법

JoVE Journal
Neuroscienze
Author Produced

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JoVE Journal Neuroscienze

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

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뮤린 망막에서 AAV 변환 효율의 정량화를위한 디지털 물방울 PCR 방법

4,151 Views

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09:50 min

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December 25, 2021

DOI:

10.3791/63038-v

DOI:

10.3791/63038-v

•

09:50 min

December 25, 2021

•

16 Views

Iskalen Cansu Topcu Okan², Mehri Ahmadian², Yesim Tutuncu², Halit Yusuf Altay², Cavit Agca²

¹Molecular Biology, Genetics and Bioengineering Program,Sabanci University, ²Nanotechnology Research and Application Center (SUNUM),Sabanci University

Tags

Digital Droplet PCR AAV Transduction Murine Retina Quantification Absolute Quantification CRISPR Gene Therapy Mitochondrial DNA Transfection Virus Purification

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