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Méthode de PCR par gouttelettes numériques pour la quantification de l’efficacité de la transduction de l’AAV dans la rétine murine
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JoVE Journal Neuroscienze
Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina
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Méthode de PCR par gouttelettes numériques pour la quantification de l’efficacité de la transduction de l’AAV dans la rétine murine

4,151 Views

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09:50 min

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December 25, 2021

DOI:

10.3791/63038-v

DOI:
10.3791/63038-v

•

09:50 min
December 25, 2021

•

16 Views
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1Molecular Biology, Genetics and Bioengineering Program,Sabanci University, 2Nanotechnology Research and Application Center (SUNUM),Sabanci University

Tags

Digital Droplet PCRAAV TransductionMurine RetinaQuantificationAbsolute QuantificationCRISPRGene TherapyMitochondrial DNATransfectionVirus Purification

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Cite this Article

Okan, I. C. T., Ahmadian, M., Tutuncu, Y., Altay, H. Y., Agca, C. Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina. J. Vis. Exp. (178), e63038, doi:10.3791/63038 (2021).

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