Rivista
/
Biochimica
/
Monstervoorbereiding en relatieve kwantificering met behulp van reductieve methylering van amines voor peptidomics-studies
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
Please note that all translations are automatically generated. Click here for the English version.

Monstervoorbereiding en relatieve kwantificering met behulp van reductieve methylering van amines voor peptidomics-studies

2,084 Views

•

08:00 min

•

November 04, 2021

DOI:

10.3791/62971-v

DOI:
10.3791/62971-v

•

08:00 min
November 04, 2021

•

10 Views
, , ,
1Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

Automatically generated

Dit artikel beschrijft een monstervoorbereidingsmethode op basis van warmte-inactivatie om endogene peptiden te behouden die post-mortem degradatie voorkomen, gevolgd door relatieve kwantificering met behulp van isotopische etikettering plus LC-MS.

Explore More Videos

Sample PreparationRelative QuantitationReductive MethylationAminesPeptidomicsProtease InactivationIsotopic LabelingNeuroblastoma CellsZebrafish BrainCellular LysateUltrafiltrationSolid-phase Extraction

Read Article

Cite this Article

Correa, C. N., Fiametti, L. O., Mazzi Esquinca, M. E., Castro, L. M. d. Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies. J. Vis. Exp. (177), e62971, doi:10.3791/62971 (2021).

Download .ris file

Copy

Share Video

.

Copy