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使用胺还原甲基化进行样品制备和相对定量，用于肽学研究

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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使用胺还原甲基化进行样品制备和相对定量，用于肽学研究

2,084 Views

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08:00 min

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November 04, 2021

DOI:

10.3791/62971-v

DOI:

10.3791/62971-v

•

08:00 min

November 04, 2021

•

10 Views

Claudia Neves Correa^1,2, Louise Oliveira Fiametti¹, Maria Eduarda Mazzi Esquinca¹, Leandro Mantovani de Castro^1,2

¹Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), ²Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

本文介绍了一种基于热灭活的样品制备方法，以保存内源性肽，避免死后降解，然后使用同位素标记和LC-MS进行相对定量。

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