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选择性清洁野生 卡诺菌线 虫以富集肠道微生物组细菌
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

选择性清洁野生 卡诺菌线 虫以富集肠道微生物组细菌

DOI:

09:47 min

August 13, 2021

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Capitoli

  • 00:04Introduction
  • 00:34Inducing Dauer Formation for Wild Nematodes on Nematode Growth Medium (NGM) Plates
  • 01:16Washing and Disinfecting the Nematodes
  • 03:12Removing the Antibiotic and SDS Solution
  • 04:08Propagate a Clean Nematode Strain
  • 05:27Intestinal Dissection and PCR Identification of Microbial Species
  • 08:17Results: The Host-microbe Interactions in the Context of the Intestines of Caenorhabditis tropicalis Strain JU1848
  • 09:16Conclusion

Summary

Traduzione automatica

野生 卡诺菌线虫 与许多微生物有关,通常在肠腔内或感染肠道。该协议详细介绍了一种利用dauer角质层的抗性来丰富无法培养的微生物定植于肠道的方法。

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