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Limpieza selectiva de nematodos silvestres de Caenorhabditis para enriquecer las bacterias del microbioma intestinal
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
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Limpieza selectiva de nematodos silvestres de Caenorhabditis para enriquecer las bacterias del microbioma intestinal

2,455 Views

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09:47 min

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August 13, 2021

DOI:

10.3791/62937-v

DOI:
10.3791/62937-v

•

09:47 min
August 13, 2021

•

4 Views
, , ,
1Department of Biology,San Diego State University, 2Institut de Biologie de l’École Normale Supérieure

Summary

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Los nematodos de Caenorhabditis silvestre están asociados con muchos microbios, a menudo en la luz intestinal o infectando el intestino. Este protocolo detalla un método para enriquecer microbios inculturables que colonizan el intestino, aprovechando la resistencia de la cutícula dauer.

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Cite this Article

Morgan, E., Longares, J. F., Félix, M., Luallen, R. J. Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria. J. Vis. Exp. (174), e62937, doi:10.3791/62937 (2021).

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