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Vrij zwevende immunostaining van muizenhersenen
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07:58 min
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October 07, 2021
DOI:
1USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics,Baylor College of Medicine, 2Department of Endocrinology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology,Baylor College of Medicine
Summary
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Dit protocol beschrijft een efficiënte en reproduceerbare aanpak voor histologische studies van muizenhersenen, waaronder perfusie, hersensectie, vrij zwevende immunostaining, weefselopbouw en beeldvorming.
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Tu, L., Zhang, N., Conde, K. M., Bean, J. C., Wang, C., Xu, Y. Free-floating Immunostaining of Mouse Brains. J. Vis. Exp. (176), e62876, doi:10.3791/62876 (2021).
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