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Free-floating Immunostaining of Mouse Brains
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07:58 min
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October 07, 2021
DOI:
1USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics,Baylor College of Medicine, 2Department of Endocrinology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology,Baylor College of Medicine
Summary
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This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.
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Tu, L., Zhang, N., Conde, K. M., Bean, J. C., Wang, C., Xu, Y. Free-floating Immunostaining of Mouse Brains. J. Vis. Exp. (176), e62876, doi:10.3791/62876 (2021).
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