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Free-floating Immunostaining of Mouse Brains
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Neurosciences
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Journal JoVE Neurosciences
Free-floating Immunostaining of Mouse Brains

Free-floating Immunostaining of Mouse Brains

DOI:
10.3791/62876-v

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07:58 min

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October 07, 2021

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, , , , ,
1USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics,Baylor College of Medicine, 2Department of Endocrinology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology,Baylor College of Medicine

Chapitres

  • 00:04Introduction
  • 00:50Perfusion
  • 02:57Cryosectioning
  • 04:06Free-Floating Wisteria Florbunda Agglutinin Staining and Anti- Arginine Vasopressin Immunostaining
  • 05:13Mounting
  • 06:00Imaging
  • 06:42Results: Double Immunofluorescent Staining of Free-Floating Mouse Brain Sections
  • 07:21Conclusion

Summary

Traduction automatique

Traduction automatique

This protocol describes an efficient and reproducible approach for mouse brain histological studies, including perfusion, brain sectioning, free-floating immunostaining, tissue mounting, and imaging.

Tags

ImmunostainingMouse BrainHistologyNeuroscience ResearchPerfusionTranscardial PerfusionFormalin FixationBrain IsolationSliding Microtome

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