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Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

DOI:
10.3791/62865-v

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09:56 min

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August 31, 2021

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, , , ,
1Neurophotonics and Mechanical Systems Biology,Institut de Ciències Fotòniques, ICFO, 2Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, 3Institut de Ciències Fotòniques, ICFO, 4Universitat Pompeu Fabra (UPF)

Capitoli

  • 00:05Introduction
  • 00:58Single-cell Preparation and Staining
  • 02:32Optical Trapping (OT) Chamber Spacing and Optical Tweezers Start-up
  • 03:58Alignment of the Optical Force Sensor
  • 05:17Performing the Nucleus Indentation Experiments
  • 07:22Results: Measurement of the Forcesand Material Properties of the Cell Nucleus Inside Zebrafish Embryo
  • 09:17Conclusion

Summary

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Here, we present a protocol to investigate the intracellular mechanical properties of isolated embryonic zebrafish cells in three-dimensional confinement with direct force measurement by an optical trap.

Tags

Optical TweezersSubcellular MechanicsCell NucleusCell PolarizationCell DifferentiationCell MigrationViscoelastic MediumCell PreparationHoechst DyeDMEMOptical TrappingOT Chamber

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