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光ピンセットを用いた閉じ込めにおける細胞下力学の直接力測定

JoVE Journal
Biologia dello sviluppo

This content is Free Access.

JoVE Journal Biologia dello sviluppo

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

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光ピンセットを用いた閉じ込めにおける細胞下力学の直接力測定

4,271 Views

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09:56 min

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August 31, 2021

DOI:

10.3791/62865-v

DOI:

10.3791/62865-v

•

09:56 min

August 31, 2021

•

25 Views

Frederic Català-Castro¹, Valeria Venturini^2,3, Santiago Ortiz-Vásquez¹, Verena Ruprecht^2,4, Michael Krieg¹

¹Neurophotonics and Mechanical Systems Biology,Institut de Ciències Fotòniques, ICFO, ²Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, ³Institut de Ciències Fotòniques, ICFO, ⁴Universitat Pompeu Fabra (UPF)

Summary

ここでは、光学トラップによる直接力測定を用いて、3次元閉じ込めで単離した胚ゼブラフィッシュ細胞の細胞内の機械的特性を調べるプロトコルを提示する。

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